Tues. Aug. 13, 2019 Pt. Whitney Juv. Geoduck resistance to stress plans

Here is a summary of new ideas for the ‘juvenile resistance to stress’ experiment following a discussion with Steven and Brent on Wed. Aug. 7:

I. chronic pH stress (2 months)

  • amb H2O w/ elev. hist.
  • amb H2O w/ amb. hist.
  • var low pH H2O with elev. hist.
  • var low pH H2O with amb. hist.
  • something like this:

II. acute stress (6 hrs, 24 hours, 48 hours?)

  • freeze samples for metabolite(omics?) and epigenetic analysis
  • respirometry?
  • Possible acute stressors:
    • temp
    • salinity
    • temp + salinity
    • lagoon water
    • desiccation

Plan for acute stress:

  1. Test one stress at a time:

  2. Test multiple stressors simultaneously:

    • I’m not sure how realistic respirometry would be for this

III. Measurements to make outside of stress trials

  1. pre-chronic stress baseline measurement
    • freeze samples for metabolite(omics?) and epigenetic analysis
    • respirometry?
  2. pre-acute stress measurement after 1 week chronic pH stress?
    • freeze samples
    • respirometry?

Proposed timeline:

References:

Summary of fall 2018-Spring 2019 samples

I also went through all the samples taken fall 2018-Spring 2019 and came up with these schematics to better understand the samples we have for possible analyses:

  1. Broodstock conditioning with constant low pH

  2. Broodstock conditioning with variable low pH

Need to discuss all this with the FFAR group on Thur Aug 15

Written on August 13, 2019