Tues. Aug. 20, 2019 Pt. Whitney Juv. Geoduck resistance to stress plans

Revised plans to address pseudo-replication concerns: img

Equipment brought to Pt. Whitney

Water chemistry

  • got apex up and running in big LRT silos
    • PH-T2 = var. low treatment
    • PH-T3 = ambient
    • didn’t actually start the treatment until wed Aug 21

Juveniles

  • changed H2 heath stack to upwell
    • moved all animals to 1mm mesh except the bottom two extra trays
    • flow now by pump (87 gal/hr), not gravity
    • Didn’t add sand
    • rinsed animals before transfering them
      • sand was black in some areas
    • reordered trays
      • Old order:
      • img
        1. T7
        2. T3
        3. T5
        4. T1
      • New order:
        1. T1
        2. T7
        3. T5
        4. T3
  • H1 stack: still fed by gravity

Manila clam setters

  • Brent added manila clams
    • to some H1 trays
    • and to 4 silos on a hang bar + a 1/2” spray bar being fed by another ambient pump (87 gal/hr)

Experimental Set-up progress

  • Determined capped spray bars would end up with uneven flow with more flowing out of the holes closest to the in-flow
    • roughly eyeballed this with the 2.5qt buckets
  • Came up with new set-up based on Mollie’s set up at Taylor
    • 3/4” PVC looped manifold with threaded adapters that flow water into silos
    • manifold sits over silos suspended by PVC hang bars
    • manifold had a port for water to flow in through

Chat with Sam about experimental setup (08/19/2019)

Feeding:

  • Sam measured algae density for 2-3 tupperware tanks every week
    • took sample in 15 ml falcon tube and used cellometer
    • measured 50K cells/ml minimum
  • Sam used dosing pumps:
    • algae 400-500Kcells (1L/min)
    • calibrate 100mL:
      • manual pump to fill line and purge b4 calib
      • press calib. use grad cylinder to fill 100mL
      • settings cycles/day: 24; set time and each hour
        • all 4 motors at a time may burn out motor so Sam staggered the dosing
        • 15 min for 2 L
        • still need to set last channel for brief time, even when not using it

water flow to mesh silos:

  • scenario 1: each conical goes to drippers by Ts and 1/4” tubing
    • dripper with orange lets out 6 Gal/hr
    • pros:
      • even flow
      • flexibility
  • scenario 2: each conical goes to spary bar
    • cons:
      • uneven flow
        • shuffle silos?

overall concerns:

  • variable low might differ between conicals
  • dosing channel might break
  • 2 replicates/ treatment cant really test the effect of conical
  • flow rate may change/conical

Next steps:

  • monitor water chem in large silos and see if it’s stable
  • purchase supplies to build manifold (spray bars, containers, etc.)
    • 10x 10ft 3/4” PVC
    • 5x 3/4” PVC ball valve (not threaded)
    • 5x 3/4” PVC ball valve (threaded)
    • 26x 7.4” x 13” x 10” 9QT plastic waste basket
    • 25x 3/4” PVC 90 degree elbow
    • 40x 3/4” PVC T
    • screw tap
    • PVC cement
  • build manifold and test it
    • here is a prettier picture of the design:
  • Sequim bay dig (Aug. 29)
    • find new home for deplanted animals
  • plan Sequim bay outplant (Aug. 30)
Written on August 20, 2019