This post is the analysis of the data collected from the full calcium assay run: https://shellytrigg.github.io/390th-post/

Assay analysis

I compiled the plate reader data into one file here: https://github.com/shellytrigg/P_generosa/blob/master/Hemolymph_Calcium_Assay/data/20201125/CompiledData.xlsx

R script here: https://github.com/shellytrigg/P_generosa/blob/master/Hemolymph_Calcium_Assay/analysis/analysis.Rmd

Rmd knitted output including stats: https://github.com/shellytrigg/P_generosa/blob/master/Hemolymph_Calcium_Assay/analysis/analysis.md

Figs

  • Standard curves for each plate:

  • calcium levels of each sample:

  • calcium levels of each sample separated by sex: