Wed. Jan 30, 2019

Geoduck Broodstock Experiment

Gonad slices sent to HCS, Inc. for histology

List of samples sent: https://drive.google.com/open?id=1Fgb5abuMR8TyiR9hbIjUW2mRg9eeoAd3pD3CdlPlqlo

Order form: https://drive.google.com/open?id=1UXb3Vc2scDMOwDsE5kWIyt8ozCF-buhj

Shipped through FedEx as hazardous goods of expected quantities, each of the 5 cassette jars contained 30 mL of 70% Ethanol (Stabilizer from PAXgene kit).

Low pH conditioning plans

Steven and I discussed stopping the treatment on Friday and combining the low pH group crates (Tank 1 and 2) into one ambient tote and Tank 3 and 4 crates into one ambient tote. This will make room for more broodstock and will hopefully rescue the low pH conditioned animals so we can get them to spawn/strip spawn. See slack discussion.

Oyster Seed Proteomics

Map the proteins to 2019 Uniprot accessions

For re-mapping the fasta to 2019 Uniprot accessions like Steven did in the past, I started the jupyter notebook linked below on Ostrich and it’s currently still running:

https://github.com/shellytrigg/OysterSeedProject/blob/master/jupyter/20190130_Cg_Giga_cont_AA.fa_BLASTP_uniprot_swprot2019.ipynb

Github desktop on Ostrich

Also got github desktop to work on Ostrich by downloading this older version that I found here. With the newest version, the graphics don’t work when you remote desktop in. But this older version works fine, probably because Ostrich is still running El Cap OS.

Written on January 30, 2019