remapping proteins sequences to 2019 Uniprot DB

reference this jupyter notebook: 20190130_Cg_Giga_cont_AA.fa_BLASTP_uniprot_swprot2019.ipynb

  1. Rebuilt the BLAST index from 2019 Uniprot DB
  2. remapped protein sequences from Steven’s file to updated Uniprot DB.

  3. reformatted BLAST output to remove pipes in protein names and contain the following fields:

fold change analysis and p-value

  • made all fold change comparisons for all time points and temperatures to time 0, and calculated p-values using chi square proportions test. Did not do anything with zero values.