May 2, 2019 Geoduck larvae at Pt. Whitney

Check on heath stack setup

  • food still dicey, sometimes the food is unevenly distributed.
    • ordered a new pump head today and they are sending some sample tubing to see if it will solve the problem (see below for more details).
    • animals looked good but some algal floculation

Algal counts

  • Matt recommended setters should get a concentration of 100K/mL, but not more than that.

  • Inflow algal counts direct from the feeding tube were:
    • H2 (ambient): 4.73 x 10^6 cells/mL
    • H1 (ambient/low pH): 4.8 x 10^6 cells/mL
    • Flow rate of the feeding tube is 10mL/13 seconds = 0.77mL/second = 46.2 mL/minute = 66,528 mL / day = 35,260 M cells/day (66,528mL * 5.3 x 10^5 cells/mL)
      • 35,260M cells get into the header tank each day
  • Outflow algal counts were:
    • H2 (ambient): 5.3 x 10^5 cells/mL
    • H1 (ambient): 3.8 x 10^5 cells/mL
    • Flow rate of the outflow is 10mL/3 seconds = 200mL mL/minute = 12,000 mL/hour = 288,000 mL/day
      • 288,000 mL * 5.3 x 10^5 cells/mL = 1.5264 x 10^11 cells/day
  • FAO manual recommends 0.4mg dry algae per mg spat per week - 10^6 Iso cells = 0.02mg, so 20M cells/week or 2.857M cells/day

  • Setter weight ~= 0.07mm^3 = 0.07mg? - H2 has ~ 52000 animals = 3.640 mg spat - H1 ambient has ~ 60,000 animals = 4.2mg spat

Conclusion

  • Ambient should be getting ~ 22.4M cells/day (2.857M cells/day * 7.84 mg total spat)
    • they are seeing 152,640 M cells/day
    • they are getting about ~6800X more than they need (152,640 M / 22.4 M)

Solutions

Water chem

Kaitlyn and Eileen ran TA on H1 water samples. TA data here and discrete chem data here

  • Still need to run poisoned samples from H1T5-T8 from 4/19 and from 5/2. We can do this next week.
Written on May 2, 2019