SHELLY A. TRIGG

University of Washington School of Aquatic and Fishery Sciences, Seattle, WA

(339)-368-0029 · strigg@uw.edu · ORCID ID: 0000-0001-6904-4149

PERSONAL SUMMARY

I am a Systems Biologist with 8+ years of experience in 'omics' technology development and application. My main interests are in research and public education outreach on how organisms’ molecular networks respond to human-influenced environmental change. I am currently a Postdoctoral Research Associate in the lab of Steven Roberts in the School of Aquatic and Fishery Sciences at the University of Washington researching how ocean acidification impacts the physiology and epigenetic variation in bivalves.

EDUCATION

Ph.D. in Biology, University of California San Diego…………………………......................2018

B.S. in Biochemistry Magna Cum Laude, Simmons College, Boston, MA…..………............2010

RESEARCH SKILLS

Lab Biology and Automation

-  High throughput sequencing library preparation

-  recombinant DNA techniques and construct design

-  PCR, qPCR, and oligo design

-  nucleic acid and protein purification

-  immuno-blotting and -precipitation

-  yeast and bacteria cell culture

-  electroporation and chemical cell transformation

-  Tecan liquid handling robots and plate readers

 

Bioinformatics and Computing

-  High throughput DNA sequencing data analysis

-  metabolomics data analysis

-  meta-analysis and data integration

-  network analysis using Cytoscape

-  R scripting and statistical analysis

-  UNIX/Linux shell scripting

-  Some Python

-  Microsoft Office applications

 

Training and Coursework

-  Mass spectrometry data processing and statistics course, UC Davis

    (2018)

-  Visiting Scientist Network Analysis Training Program, Broad Institute (2016)

-  NSF Graduate Data Science Workshop, University of Washington (2015)

-  Graduate Biostatistics, UC San Diego (2013)

-  Tecan EVO Training Course (2012)

RESEARCH EXPERIENCE

University of Washington School of Aquatic and Fishery Sciences, Roberts Lab, Seattle, WA

Postdoctoral Research Associate…...………………......................................Sept 2018 - Present

Advisor: Steven Roberts Ph.D.

·  Lead research efforts on how environmental change, like ocean acidification, impacts the physiology and epigenetic variation in marine invertebrates, particularly bivalves

NOAA Northwest Fisheries Science Center, Conservation Biology/Genetics Division, Seattle, WA

Volunteer…...……………………..………………......………………….......Jun 2018 - Present

NSF Graduate Research Internship Program Fellow…...………………......Jun 2017 - May 2018

Project title:  Understanding the physiological and genetic impacts of ocean acidification on marine invertebrates

Advisors: Paul McElhany Ph.D. and Krista Nichols Ph.D.

·  Performed quality control, statistical, and physiological pathway analyses of metabolomics datasets from juvenile Dungeness crab exposed to lab simulated ocean acidification conditions

·  Carried out time-course high throughput ocean acidification exposure experiments on Dungeness crab larvae

·  Prepared whole genome sequencing libraries for the Dungeness crab genome project

·  Did field collection and laboratory rearing of Dungeness crab larvae and adults

Salk Institute for Biological Studies, Ecker Lab Genomic Analysis Section, La Jolla, CA

NSF Graduate Research Fellow…………………………………….………...….........2014 - 2018

Graduate Student Researcher…………………………………….………….…….......2013 - 2018

Ph.D. dissertation:  High-resolution molecular networks from novel 'omics' approaches elucidate survival strategies in organisms from land to sea

Advisor: Joseph Ecker, Ph.D.

·  Developed a next-generation DNA sequencing assay (CrY2H-seq) for high throughput protein-protein interaction screening and coded an analysis pipeline to identify and quantify interactions from large sequence datasets

·  Generated a comprehensive protein-protein interactome map for Arabidopsis by creating high complexity clone libraries and screening libraries with CrY2H-seq

·  Validated protein interaction networks found with CrY2H-seq by performing experimental and computational quality control, in-depth network analysis, data integration, and functional annotation of protein

Research Assistant II…………………………………….………...…..........................2012 - 2013

Research Assistant I…………………………………….………...…............................2011 - 2012

Dana Farber Cancer Institute, Center for Cancer Systems Biology, Vidal Lab, Boston, MA

Research Technician I…………………………………….………...…........................2010 - 2011

·     Generated comprehensive human protein-protein interactome using high throughput yeast two-hybrid screening, protein complementation assay, and well-nucleic-acid-programmable-protein-array (wNAPPA) assay

·     Generated interactomes using high throughput yeast two-hybrid screening of autism genes and human isoforms against a human ORFeome collection

Research Trainee…………………………………….………...…..................Jan 2010 - Aug 2010

·     Constructed arrayed collections of Kaposi’s Sarcoma and Epstein-Barr viral ORFs by high throughput gateway cloning for yeast two-hybrid screening against a human ORFeome to define potential cancer driver and passenger proteins

Simmons College, Department of Chemistry, Roecklein-Canfield Lab, Boston, MA

Undergraduate Researcher…………………………………….………...…................2009 - 2010

·    B.S. Honors Thesis: The effectiveness of the GST fusion system for isolating Epstein-Barr viral thymidine kinase, a potential target for antiviral antitumor therapies

PUBLICATIONS AND MANUSCRIPTS

Shelly A. Trigg, M Maher, D Perez, DS Busch, P McElhany, and KM Nichols. Uncovering mechanisms of global ocean change effects on Dungeness crab (Cancer magister) through metabolomics analysis. (In preparation)

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, A Bartlett, R Castanon, A Goubil, J Feeney, R O’Malley, SC Huang, ZZ Zhang, M Galli, and JR Ecker (2017) CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping. Nat Methods. 14(8):819-825.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, A Bartlett, R Castanon, A Goubil, J Feeney, R O’Malley, SC Huang, ZZ Zhang, M Galli, and JR Ecker (2017) CrY2H-seq interacome screening. Protoc. Exch. doi:10.1038/protex.2017.058.

 

X Yang, [15 others], Shelly A. Trigg, [20 others], and M Vidal. (2016) Widespread expansion of protein interaction capabilities by alternative splicing. Cell. 164(4):805-817.

 

T Rolland, [53 others], Shelly A. Trigg, [14 others], and M Vidal. (2014) A proteome-scale map of the human interactome network. Cell. 159(5):1212-1226.

 

R Corominas, [8 others], Shelly A. Trigg, [18 others], M Vidal, and LM Iakoucheva. (2014) Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism. Nat commun. 5:3650. 

 

O Rozenblatt-Rosen, [36 others], Shelly Wanamaker, [13 others], and M Vidal. (2012) Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins.  Nature. 487(7408):491-495.

FIRST AUTHOR CONFERENCE PRESENTATIONS

Shelly A. Trigg, P McElhany, M Maher, D Perez, DS Busch, and KM Nichols. (Mar 2018) Uncovering mechanisms of global ocean change effects on Dungeness crab through metabolomics analysis. NOAA NWFSC 6th Science Symposium, Seattle, WA, oral.

 

Shelly A. Trigg, M Maher, D Perez, DS Busch, P McElhany, and KM Nichols. (Feb 2018) Uncovering mechanisms of global ocean change effects on Dungeness crab through metabolomics analysis. Ocean Sciences Meeting, Portland, OR, poster.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, CS Huang, ZZ Zhang, M Galli, and JR Ecker (Sept 2016) ProCREate: A New Assay for Generation of Large-Scale Protein-Protein Interaction Maps, HHMI Scientific Meeting, Janelia Research Campus, Ashburn, VA, poster.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, CS Huang, ZZ Zhang, M Galli, and JR Ecker (Sept 2016) ProCREate: A New Assay for Generation of Large-Scale Protein-Protein Interaction Maps, UCSD Biology annual retreat, Lake Arrowhead, CA, poster.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, CS Huang, ZZ Zhang, M Galli, and JR Ecker (Mar 2016) Next-Generation Protein Interactomes for Plant Systems Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for Bioenergy Meeting, Tysons Corner, VA, poster.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, CS Huang, ZZ Zhang, M Galli, and JR Ecker (October 2015) The ProCREate interactome mapping technique reveals crosstalk and directionality between Arabidopsis regulatory networks, Salk Science at the Seaside, La Jolla, CA, poster and mini presentation. Peoples Choice Award for best mini presentation.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, CS Huang, ZZ Zhang, M Galli, and JR Ecker (Sept 2015) ProCREate: A New Assay for Generation of Large-Scale Protein-Protein Interaction Maps, UCSD Biology annual retreat, Lake Arrowhead, CA, poster.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, CS Huang, M Galli, and JR Ecker (Aug 2015) Finding biological relevance in large-scale protein network studies, NSF Data Science Workshop, Seattle, WA, poster.

 

Shelly A. Trigg, RM Garza, A MacWilliams, JR Nery, J Reyna, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, CS Huang, M Galli, and JR Ecker (Feb 2015) Next-Generation Protein Interactomes for Plant Systems Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for Bioenergy Meeting, Tysons Corner, VA, poster.

 

Shelly A. Trigg, A MacWilliams, JR Nery, A Bartlett, R Castanon, A Goubil, J Feeney, R OMalley, M Galli, and JR Ecker (Feb 2014) Next-Generation Protein Interactomes for Plant Systems Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for Bioenergy Meeting, Arlington, VA, poster.

 

Shelly A. Trigg, J Feeney, JR Nery, A Bartlett, R Castanon, A Goubil, R OMalley, M Galli, and JR Ecker (Feb 2013) Next-Generation Protein Interactomes for Plant Systems Biology and Biomass Feedstocks Research, USDA-DOE Plant Feedstock Genomics for Bioenergy Meeting, Bethesda, MD, poster.

ACADEMIC SERVICE

Teacher training coursework

Evidence-Based Teaching and Learning in Biological Sciences, UC San Diego                      Winter 2017

Introduction to College Biology Education, UC San Diego                                                    Spring 2016

 

Teaching experience

Head Instructional Assistant                                                                                       

Regulation of Eukaryotic Gene Expression, UC San Diego                                                   Winter 2017

Instructional Assistant                                                                                               

Genomics Research Initiative Laboratory, UC San Diego                                                       Spring 2016

Biochemical Techniques Laboratory, UC San Diego                                                                  Fall 2014 

Graduate Student Instructor

Quantitative Biology graduate seminar, UC San Diego                                                          2015 - 2016

Undergraduate Teaching Assistant                                                                                                   

Biochemistry II, Simmons College                                                                                      Spring 2010

Organic Chemistry I, Simmons College                                                                                Spring 2009

Organic Chemistry II, Simmons College                                                                                  Fall 2008

General Chemistry, Simmons College                                                                               Summer 2008

 

Mentoring Experience

NOAA Hollings Scholar undergraduate student Akofa Kekeh                                            Summer 2017

Biology Department graduate peer mentor committee chair                                                  2015 - 2016

Graduate peer mentor of graduate student Eileen Gonzales                                                   2015 - 2016

Graduate peer mentor of graduate student Natasha Slepak                                                    2014 - 2015

EDUCATION OUTREACH

National Marine Sanctuaries ocean acidification multimedia toolkit development                   2017 - 2018

Salk Institute Education Outreach plant biology video media development                                       2016

Salk Institute AAAS New Frontiers in Science Education curriculum developer                    2014 - 2017

UC San Diego Undergraduate Biology Showcase poster judge                                              2015 - 2018

Reuben H. Fleet Science Center #2Scientists program                                                           2014 - 2016

Association for Women in Science (AWIS) San Diego outreach committee member              2012 - 2016

Expanding Your Horizons San Diego AWIS presenter                                                         2013 - 2015

AWIS San Diego undergraduate career panel coordinator                                                                 2014

Albert Einstein Academy Family Science Night presenter                                                               2014

ACS Chemistry Expo AWIS presenter, San Diego                                                                         2013

Greater San Diego Science Festival Expo Day presenter                                                       2013 - 2014

Greater San Diego Science Festival poster judge                                                                  2013 - 2014

AWARDS

NSF Graduate Research Internship                                                                                      2017 - 2018

Excellence in Teaching Award, UC San Diego Division of Biological Sciences                                2016

Salk Institute Annual Retreat poster presentation People’s Choice Award                                        2015

NSF Graduate Research Fellowship                                                                                     2014 - 2018

Rookie of the Year Award, Association for Women In Science, San Diego                                      2013

Allen Douglass Bliss Award in Chemistry for academic achievement and promise in the field          2010

PROFESSIONAL MEMBERSHIPS

The Ocean Society

American Association for the Advancement of Science

Association of Women in Science

Tri Beta Biological Honors Society

Sigma Xi Biological Honors Society