Notes from Feb. 14 spawn:
2M swimmers from 15M eggs (13% hatch rate) from Oct. 2019 harvested brood
1.5M swimmers from 4M eggs from tank 2 volitional spawn
0.5M swimmers from residual eggs from side experiment without KCl from Oct. 2019 harvested brood
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Friday. Feb. 14, Point Whitney Strip Spawn
Matt, Brent, Nate (from hatchery in Lilliwaup?), and Josh present
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Wed. Jan. 22, 2020 KCl in Geoduck Strip Spawn
Does priming with KCl matter?
This was a small scale experiment performed to determine if fertilization can be achieved without KCl priming.
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Tues. Jan. 21, 2020 Pt Whitney broodstock conditioning
Strip spawn prep
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Tues. Jan. 7, 2020 Geoduck methylation analysis on 5x cov. destranded CpG data
Steven generated destranded (“merged”) coverage files from Bismark .cov output.
Files are here: https://osf.io/yem8n/files/ under “bismark_coverage_files” under “merged_tab”.
I believe Steven did this using this notebook:
40-cov2cyt-zero.ipynb
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